Anti-Oxidant Effect of Lycium Barbarum Polysaccharides on the Retina of Streptozotocin-induced Diabetes Rats

 

Abstract

Aim: To investigate the effect of Lycium barbarum polysaccharides (LBP) on the oxidation damage of retina of streptozotocininduced diabetes rats.

Methods: The diabetes rats were induced by injection streptozotocin intraperitoneally. LBP was intragastrically administered to the rats of the LBP group. Body weight and blood glucose were measured every four weeks. In the 24th week, the SOD and MDA of retina were measured, the expression of VEGF mRNA of retina was tested by RT-PCR, the ultrastructure was observed by transmission electron microscopy.

Results: The body weight decreased and the blood glucose increased in DM group and LBP group (P<0.01, VS control group). The body weight and blood glucose is the same level between DM group and LBP group (body weight P=0.503, blood glucose P=0.984). The SOD in DM group decreased and the MDA increased (P<0.05, VS control group). The SOD in LBP group increased and the MDA decreased (P<0.05, VS DM group). The expression of VEGF mRNA was highest in DM group and lowest in control group, and decreased in LBP group (P<0.05, VS DM group). The ultrastructure changes occur in almost all retinal nerve tissues in DM group, as represented by the number and morphology of mitochondria. A small amount of mild mitochondrial changes could be observed in the endochylema of bipolar and Müller cells in LBP group.

Conclusion: LBP can reduce the oxidative damage, alleviate the pathological changes in mitochondria, prevent the apoptosis of nerve cells and block the progression of diseases to the vascular tissue.

Keywords: Lycium Barbarum Polysaccharides; Diabetes; Anti-Oxidant; Diabetic Retinopathy

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